Arabidopsis thaliana

This Website describes the labs of genetics of the reference plant Arabidopsis thaliana.

Individuals in our labs now involved in this job Todd Michael (Salk), Todd Mockler (Salk), Jon Werner (Salk), Samuel Hazen (Scripps), Chris Schwartz (Madison), Kazunari Nozue (Davis), Norman Warthmann (MPI), Janne Lempe (MPI), Yasushi Kobayashi (MPI), Anandita Singh (MPI), Sureshkumar Balasubramanian (MPI). Olivier Loudet has his own set in France.

Arabidopsis is nicely suited to the analysis of natural version because small heterozygosity is present in natural populations, and as it’s a cosmopolitan species for which shares from a number of locales across the Northern hemisphere are easily obtainable. Considering that the genome sequence is finished, positional cloning of quantitative trait loci (QTL) has to become a lot more viable. Quantitative genetic approaches in Arabidopsis have been initiated by Dr. Maarten Koorrnneef along with his coworkers (Alonso-Blanco and Koornneef, 2000).

A couple of decades back, we started to characterize the level of variation in light sensitivity and lively behaviour in natural populations of Arabidopsis (Maloof et al., 2001; Jon Werner et al., unpublished). We also have started to map QTL that influence both of these traits (Borevitz et al., 2002). We’ve already identified the shift that’s responsible for its diminished far-red reaction in one definite accession (Maloof et al., 2001). We have cloned one QTL affecting sensitivity and yet another one. Among those replies we anticipate from such studies is whether there’s a prejudice in the types of enzymes like genes encoding structural proteins, signal transduction elements or enzymes.

Additionally, we’re producing new genomic and computational tools for the analysis of qualitative traits, for example high-throughput genotyping with oligonucleotide processors. Previously, we’ve used this method together with PCR amplification of genomic DNA surrounding human markers (Nordborg et al., 2002). More recently, we’ve started to utilize lead hybridization of genomic DNA into microarrays (Borevitz et al, 2003), like the methods created for yeast. We are utilizing microarrays to describe variation in gene content, expression levels and genome-wide haplotype patterns.
To describe extra QTL, we’ve started to create several collections of Recombinant Inbred Lines (RILs) by crossing distinct accessions into the Columbia benchmark strain. Up-to-date info regarding the standing of our RILs are located under”Genetic Resources”. Places are generated from the All-natural consortium and from Alan Lloyd’s laboratory in the University of Texas, Austin. Formerly sets are located at NASC stock centres or the ABRC. Magnus Nordborg in USC has picked a pair of 96 accessions to get genome-wide polymorphism evaluation.

A so far mostly untapped source of genetic data is that the genetic variant that exists in natural populations. The reason for the belief is that may signify variations which are characterized as a change-of-function instead of reduction – or – gain-of-function alleles, and that are difficult or impossible to discover by mutagenesis from the lab. Alleles that are Organic are likely to influence traits within a method. Additionally, comprehending genetic variant has the capacity to notify us concerning changes which are important from plants’ surroundings.