Hecaton: reliably detecting copy number variation in plant genomes using short read sequencing data.

Hecaton: reliably detecting copy number variation in plant genomes using short read sequencing data.

Copy number variation (CNV) is believed to actively contribute to adaptive evolution of plant species. While many computational algorithms can be found to detect copy number variation from complete genome sequencing datasets, the everyday complexity of plant knowledge possible introduces false constructive calls.To allow dependable and complete detection of CNV in plant genomes, we developed Hecaton, a novel computational workflow tailor-made to vegetation, that integrates calls from a number of state-of-the-art algorithms by way of a machine-learning strategy.

In this paper, we exhibit that Hecaton outperforms present strategies when utilized to short read sequencing knowledge of Arabidopsis thaliana, rice, maize, and tomato. Moreover, it accurately detects dispersed duplications, a sort of CNV generally discovered in plant species, in distinction to a number of state-of-the-art instruments that erroneously signify such a CNV as overlapping deletions and tandem duplications. Finally, Hecaton scales nicely in phrases of reminiscence utilization and working time when utilized to short read datasets of domesticated and wild tomato accessions.Hecaton offers a sturdy methodology to detect CNV in vegetation. We count on it to be of speedy curiosity to each utilized and basic analysis on the connection between genotype and phenotype in vegetation.

Horticultural vegetation play varied and demanding roles for people by offering fruits, greens, supplies for drinks, and natural medicines and by appearing as ornamentals. They have additionally formed human artwork, tradition, and environments and thereby have influenced the existence of people. With the arrival of sequencing applied sciences, there was a dramatic improve in the number of sequenced genomes of horticultural plant species in the previous decade. The genomes of horticultural vegetation are extremely various and complicated, typically with a excessive diploma of heterozygosity and a excessive ploidy attributable to their lengthy and complicated historical past of evolution and domestication.

Here we summarize the advances in the genome sequencing of horticultural vegetation, the reconstruction of pan-genomes, and the event of horticultural genome databases. We additionally focus on previous, current, and future research associated to genome sequencing, knowledge storage, knowledge high quality, knowledge sharing, and knowledge visualization to offer sensible steering for genomic research of horticultural vegetation. Finally, we suggest a horticultural plant genome undertaking in addition to the roadmap and technical particulars towards three targets of the undertaking.

Draft genome sequence of Bacillus paralicheniformis TRQ65, a organic management agent and plant growth-promoting bacterium remoted from wheat (Triticum turgidum subsp. durum) rhizosphere in the Yaqui Valley, Mexico.

 

The pressure denominated TRQ65 was remoted from wheat (Triticum turgidum subsp. durum) business fields in the Yaqui Valley, Mexico. Here, we report its draft genome sequence, which offered ~ 4.5 million bp and 45.5% G + C content material. Based on the cutoff values on species delimitation established for common nucleotide identification (> 95 to 96%), genome-to-genome distance calculator (> 70%), and the reference sequence alignment-based phylogeny builder methodology, TRQ65 was strongly affiliated to Bacillus paralicheniformis. The fast annotation using subsystem expertise server revealed that TRQ65 accommodates genes associated to osmotic, and oxidative stress response, in addition to auxin biosynthesis (plant development promotion traits).

In addition, antiSMASH and BAGEL revealed the presence of genes concerned in lipopeptides and antibiotic biosynthesis. The operate of these annotated genes was validated at a metabolic stage, observing that pressure TRQ65 was in a position to tolerate saline (91.0%), and water (155.0%) stress circumstances, in addition to producing 28.8 ± 0.9 µg/mL indoles. In addition, pressure TRQ65 confirmed development inhibition (1.6 ± 0.Four cm inhibition zone) towards the causal agent of wheat spot blotch, Bipolaris sorokiniana.

Finally, plant-microbe interactions assays affirm the power of pressure TRQ65 to manage wheat development, exhibiting a big increment in shoot top (26%), root size (40%), shoot dry weight (48%), stem diameter (55%), and biovolume index (246%). These findings present insights for future agricultural research of this pressure. Parasitic vegetation in the genus Striga, generally generally known as witchweeds, trigger main crop losses in sub-Saharan Africa and pose a menace to agriculture worldwide. An understanding of Striga parasite biology, which may result in agricultural options, has been hampered by the dearth of genome info.

Here, we report the draft genome sequence of Striga asiatica with 34,577 predicted protein-coding genes, which displays gene household contractions and expansions which might be per a three-phase mannequin of parasitic plant genome evolution. Striga seeds germinate in response to host-derived strigolactones (SLs) after which develop a specialised penetration construction, the haustorium, to invade the host root. Considering that outcrossing pear is an angiosperm species that includes very excessive heterozygosity, our methodology for quickly phasing genome assemblies is probably relevant to a number of yet-unsequenced outcrossing angiosperm species in nature.

Hecaton: reliably detecting copy number variation in plant genomes using short read sequencing data.

Single-pollen-cell sequencing for gamete-based phased diploid genome meeting in vegetation.

 

Genome assemblies from diploid organisms create mosaic sequences alternating between parental alleles, which might create faulty gene fashions and different issues. In animals, a well-liked technique to generate haploid genome-resolved assemblies has been the sampling of (haploid) gametes, and the arrival of single-cell sequencing has additional superior such strategies. However, a number of challenges for the isolation and amplification of DNA from plant gametes have restricted such approaches in vegetation.

SequaGel Sequencing System 2.2L Kit

NAT1138 EACH
EUR 211

MicroElute PCR Clean Up Kit (50prep)

FAMPK-001 50 preps
EUR 126

MicroElute PCR Clean Up Kit (200prep)

FAMPK-001-1 200 preps
EUR 187

PCR Clean-Up Mini Kit (50prep)

FAPCK-001 50 preps
EUR 120

PCR Clean-Up Mini Kit (200prep)

FAPCK-001-1 200 preps
EUR 169

PCR Clean-Up Mini Kit (300prep)

FAPCK-001-2 300 preps
EUR 189

FavorPrep Genomic DNA Clean-Up Kit (50prep)

FAGDC-001 50 preps
EUR 129

FavorPrep Genomic DNA Clean-Up Kit (200prep)

FAGDC-001-1 200 preps
EUR 204

96-well PCR Clean-Up Kit (1 prep)

FACKE-96001 1 prep
EUR 158

96-well PCR Clean-Up Kit (2 prep)

FACKE-96002 2 preps
EUR 213

96-well PCR Clean-Up Kit (4 prep)

FACKE-96004 4 preps
EUR 317

AXYPREP MAG PCR CLEAN UP KIT- 1 ML - (SAMPLE SIZE)

MAG-PCR-CL-1 1/pk
EUR 58
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

AXYPREP MAG PCR CLEAN UP KIT- 5 ML - 110 PREPS

MAG-PCR-CL-5 1/pk
EUR 146
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

AXYPREP MAG PCR CLEAN UP KIT- 50 ML - 1110 PREPS

MAG-PCR-CL-50 1/pk
EUR 579
Description: Bioscience Mag Beads; Magnetic PCR Clean Up

96-well GE/PCR Clean Up Purification Kit (1 plates)

FAPKE-96001 1 prep
EUR 146

96-well GE/PCR Clean Up Purification Kit (2 plates)

FAPKE-96002 2 preps
EUR 188

96-well GE/PCR Clean Up Purification Kit (4 plates)

FAPKE-96004 4 preps
EUR 274

Chymotrypsin for Sequencing grade

C4001-010 4x25ug
EUR 313

Chymotrypsin for Sequencing grade

C4001-100 100ug
EUR 286

sequencing unit 33x45 cm

ESEQ1100-SYS ea
EUR 1535

Trypin for Mass & Sequencing

T9600-025 25ug
EUR 145

Trypin for Mass & Sequencing

T9600-100 100ug
EUR 219

Trypin for Mass & Sequencing

T9600-112 12x100ug
EUR 1657

Trypin for Mass & Sequencing

T9600-400 4x100ug
EUR 651

Individual Reaction Mix

G065-X 200 reactions
EUR 167

2X Reaction Buffer

1068-20
EUR 185

2X Reaction Buffer

1068-80
EUR 294

VAHTS DNA Clean Beads

N411-01 5 ml
EUR 216

VAHTS DNA Clean Beads

N411-02 60 ml
EUR 529

VAHTS DNA Clean Beads

N411-03 450 ml
EUR 2546

VAHTS RNA Clean Beads

N412-01 5 ml
EUR 239

VAHTS RNA Clean Beads

N412-02 40 ml
EUR 413

VAHTS RNA Clean Beads

N412-03 450 ml
EUR 4050

Zenoquick DNA Clean & Concentrator-5

Z2001-050 50 prpe
EUR 180

Individual Reaction Mix 1

G065-1 200 reactions
EUR 167

Individual Reaction Mix 10

G065-10 200 reactions
EUR 167

Individual Reaction Mix 11

G065-11 200 reactions
EUR 167

Individual Reaction Mix 12

G065-12 200 reactions
EUR 167

Individual Reaction Mix 2

G065-2 200 reactions
EUR 167

Individual Reaction Mix 3

G065-3 200 reactions
EUR 167

Individual Reaction Mix 4

G065-4 200 reactions
EUR 167

Individual Reaction Mix 5

G065-5 200 reactions
EUR 167

Individual Reaction Mix 6

G065-6 200 reactions
EUR 167

Individual Reaction Mix 7

G065-7 200 reactions
EUR 167

Individual Reaction Mix 8

G065-8 200 reactions
EUR 167

Individual Reaction Mix 9

G065-9 200 reactions
EUR 167

amfiXpand 2x Reaction Buffer

P0333-001 1ml
EUR 101

amfiFusion 2x Reaction Buffer

P0343-001 1ml
EUR 104

RANGER Reaction Buffer, 5x

BIO-37114 3 x 1.25ml Ask for price

MyFi Reaction Buffer, 5x

BIO-37115 3 x 1.25ml Ask for price

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 12 Rxns
EUR 480

West-Q Femto Clean ECL Solution

W3680-010 100ml
EUR 336

West-Q Femto Clean ECL Solution

W3680-020 200ml
EUR 493

West-Q Femto Clean ECL Solution

W3680-040 400ml
EUR 631

T-Pro Aqua EZ Clean (M)

JT90-R001M 500ml/BT
EUR 332

T-Pro Aqua EZ Clean (S)

JT90-R001S 100ml/BT
EUR 151

Kinase Reaction Buffer Concentrate, 60ML

X104-60ML 60ML
EUR 122

amfiEco Taq Reaction Buffer(2X)

P0703-001 1ml
EUR 84

amfiEco Taq Reaction Buffer(2X)

P0703-005 5ml
EUR 178

Hi-Fi Reaction Buffer, 5x

BIO-37110 2 x 1.5ml Ask for price

MyTaq reaction Buffer Colourless, 5x

BIO-37111 4 x 1ml Ask for price

MyTaq Reaction Buffer Red, 5x

BIO-37112 4 x 1ml Ask for price

ProtoBlock System

NAT1004 1KIT
EUR 102

Universal platform with adjustable bars, 2 x 1000mL flasks or 4 x 500mL flasks or 4 x 250mL flasks for UniPact?

ACT-USI-UP
EUR 532

amfiRivert Reverse Transcriptase Reaction Buffer(5X)

A1203-001 1ml
EUR 88

Up RNA Extraction Kit

20-abx098087
  • EUR 258.00
  • EUR 105.00
  • 100 ml
  • 3 ml

UP-X898MD thermal printer

2140202 1unit
EUR 2384

Rabbit UP ELISA Kit

ERTU0018 96Tests
EUR 521

Rat UP ELISA Kit

ERU0018 96Tests
EUR 521

Mouse UP ELISA Kit

EMU0018 96Tests
EUR 521

Porcine UP ELISA Kit

EPU0018 96Tests
EUR 521

Goat UP ELISA Kit

EGTU0018 96Tests
EUR 521

Human UP ELISA Kit

EHU0018 96Tests
EUR 521

Bovine UP ELISA Kit

EBU0018 96Tests
EUR 521

Canine UP ELISA Kit

ECU0018 96Tests
EUR 521

Anserine UP ELISA Kit

EAU0018 96Tests
EUR 521

Fast Mutagenesis System

20-abx098073
  • EUR 509.00
  • EUR 592.00
  • 10 rxns
  • 20 rxns

CCD cooling system

3600100 1unit
EUR 4914

photo documentation system

VLBPCX4-20M ea
EUR 6352

photo documentation system

VLBPCX4-20MX ea
EUR 6774

photo documentation system

VLDP-HOOD ea
EUR 5084

photo documentation system

VLDP17-26M ea
EUR 8126

photo documentation system

VLDP17-26MX ea
EUR 8464

data acquisition system

D230 ea
EUR 1704

CometAssay Electrophoresis System

4250-050-ES 1 Kit
EUR 2950.8

Cometslides Rack System

4252-040-02 1 Each
EUR 213.8

AEC coloring system

CT356 10-plate
EUR 141

Signal Guard™ HRP reaction stopping solution

11020 0.5 ml
EUR 132

WarmStart Enzyme Modification Kit, 0.1 mg Reaction

29053 0.1mg
EUR 867
Description: Minimum order quantity: 1 unit of 0.1mg

WarmStart Enzyme Modification Kit, 0.5 mg Reaction

29053-T 0.5mg
EUR 266
Description: Minimum order quantity: 1 unit of 0.5mg

HotStart Enzyme Modification Kit, 0.1 mg Reaction

29054 0.1mg
EUR 867
Description: Minimum order quantity: 1 unit of 0.1mg

HotStart Enzyme Modification Kit, 0.5 mg Reaction

29054-T 0.5mg
EUR 266
Description: Minimum order quantity: 1 unit of 0.5mg

Human Proteinuria,UP ELISA Kit

CN-04343H1 96T
EUR 436

Human Proteinuria,UP ELISA Kit

CN-04343H2 48T
EUR 287

WESTSAVE Up (Western Blotting Substrate)

LF-QC0101 1 kit
EUR 151
Description: Abfrontier WESTSAVE UpTM (Western Blotting Substrate) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase(HRP) labeled antibodies.

Human Proteinuria,UP ELISA Kit

201-12-1084 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Myoglobin (Up-converting Phosphor Technology)

abx095254-80Units 80 Units
EUR 954

Procalcitonin (Up-converting Phosphor Technology)

abx095257-80Units 80 Units
EUR 1191

Up-converting Phosphor immunoassay analyzer

abx095263-1Machine 1 Machine
EUR 6105

Up Plus RNA Extraction Kit

20-abx098093
  • EUR 370.00
  • EUR 105.00
  • 100 rxns
  • 2 rxns

WSE-5400-UP Printgraph Classic

2305403 1unit
EUR 10149
Description: M ai n uni t , Cont r ol , U V, Ther mal Pr i nt er

Human Proteinuria(UP)ELISA Kit

GA-E1100HM-48T 48T
EUR 289

Human Proteinuria(UP)ELISA Kit

GA-E1100HM-96T 96T
EUR 466

Guinea Pig UP ELISA Kit

EGU0018 96Tests
EUR 521

Human Proteinuria(UP)ELISA Kit

QY-E02063 96T
EUR 361

Human Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E01M0044-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E01M0044-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E01M0044-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E03M0044-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E03M0044-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E03M0044-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E02M0044-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E02M0044-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E02M0044-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E08M0044-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E08M0044-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E08M0044-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E06M0044-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E06M0044-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E06M0044-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E07M0044-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E07M0044-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E07M0044-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E04M0044-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E04M0044-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E04M0044-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E09M0044-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E09M0044-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Mixed Lymphocyte Reaction Blocking Factors ELISA kit

E09M0044-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Mixed Lymphocyte Reaction Blocking Factors in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-560 20 rcs
EUR 102

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-562 100 rcs
EUR 302

First.Strand cDNA PLUS Synthesis Kit (20 µl / reaction)

105-564 1000 rcs
EUR 2265

FractionPREP Cell Fractionation system

55R-1359 50 assays
EUR 758
Description: Cell Fractionation system for use in the research laboratory

Fast MultiSite Mutagenesis System

abx098861-10rxns 10 rxns
EUR 690

amfiRivert Reverse Transcription System

R5200-050 50 rxns
EUR 388

myGel Mini Electrophoresis System

BM0286 System
EUR 618

96-Well Vacuum System

SD5011 1 UNIT
EUR 324.05

modular system 10x10 cm

EVS1100-MULTI ea
EUR 1197

modular system 20x10 cm

EVS1200-MULTI ea
EUR 1451

modular system 20x20 cm

EVS1300-MULTI ea
EUR 1958

vertical system 10x10 cm

EVS3100-SYS ea
EUR 775

vertical system 20x10 cm

EVS3200-SYS ea
EUR 1451

vertical system 20x20 cm

EVS3300-SYS ea
EUR 1704

mini system 8x10 cm

EHS1050-SYS ea
EUR 352

mini system 7x8 cm

EHS3100-SYS ea
EUR 437

cfMAX cfDNA Isolation System

CFMAX100A-1 1 Kit
EUR 479

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 176

Here, we mixed a brand new strategy for pollen protoplast isolation with a single-cell DNA amplification approach after which used a “barcoding” bioinformatics technique to include haploid-specific sequence knowledge from 12 pollen cells, finally enabling the environment friendly and correct phasing of the pear genome into its A and B haploid genomes. Beyond revealing that 8.12% of the genes in the pear reference genome characteristic mosaic assemblies and enabling a beforehand inconceivable evaluation of allelic impacts in pear gene expression, our new haploid genome assemblies present high-resolution details about recombination throughout meiosis in pollen.